Microplate Luminometer Maintenance
100
Proceed as follows:
1. Wash with deionized or distilled water
1.1 Use deionized or distilled water to remove the reagents
remaining in the injection system.
1.2 Place the priming container on the microplate loading area.
1.3 Start the cleaning protocol and initialize the injectors.
1.4 Click the <Priming> button and select the injector(s) you
want to clean.
1.5 Select a volume of 150 µl and 20 injection cycles (strokes).
1.6 Click <OK> to start the wash process.
1.7 Repeat steps 1.4 - 1.6.
2. 1
st
Cleaning Step:
Cleaning the Injector(s) with 0.1N NaOH
2.1 Remove front cover of injector unit to watch the syringe
movement.
2.2 Fill the reagent bottles and injector tubing with 0.1N NaOH.
2.3 Click the <Priming> button and select the injector(s) you
want to clean.
2.4 Select a volume of 150 µl and 20 injection cycles (strokes).
2.5 Click <OK> to start the cleaning process.
2.6 Repeat steps 2.3 – 2.5
2.7 Start a measurement by clicking the green <Start> button;
click the red <Stop> button when the syringe has reached
the extended position, and let the syringe stay in this posi-
tion for about 10 minutes.
2.8 Wash the syringe with deionized or distilled water using the
priming routine (20 cycles (strokes) with 150 µl each).
3. 2
nd
Cleaning Step: Cleaning with 0.1N HCL
3.1 Fill the syringe with 0.1N HCL and leave this solution for
about 10 minutes in the syringe (same as for cleaning with
NaOH, steps 2.2 – 2.6).
3.2 Start a measurement by clicking the green <Start> button;
click the red <Stop> button when the syringe has reached
the extended position, and let the syringe stay in this posi-
tion for about 10 minutes.
3.3 Then wash the syringe with deionized or distilled water,
using the priming routine (20 cycles (s trokes ) with 15 0 µl each).
