Microplate Luminometer System Description
10
❏ Turn down the frame and push it shut until it snaps into place
(audible click!). The microplate is held in place by the fitting
piece (Figure 2-4: (a)), by the spring on the frame at the
rear between the hinges (b) and by the spring-loaded support
tray (c).
❏ Close the instrument door. Push it shut until it clicks into
place with an audible noise. Then, the instrument is closed
light-tight.
2.2.3 Measurement Chamber
The measurement chamber is separated from the microplate
loading compartment. It is sealed light-tight only when the serv-
ice door has been closed properly.
For measurement, the transport unit, with the microplate, will
move into the measurement chamber, so that the well to be
measured is exactly positioned below the photomultiplier. The
wells are measured column by column (A1 to H1, A2 to H2 ...
A12 to H12).
For information on the position of the injector tips please read
section 2.2.6. 0.
The measurement chamber is not accessible to the user. Dirt in
the measurement chamber can therefore be removed only by
authorized service engineers.
2.2.4 Light Detector
The light detector measures visible light in the range from 300 to
650 nm. The photons emitted by the sample are converted into
electrons and multiplied by a photomultiplier. The single pulses,
which are digitally counted, are directly proportional to the emit-
ted light quantity.
The light emitted by the samples can only be read from above
and is – since the photomultiplier, for practical reasons (flat in-
strument design), is installed horizontally – reflected by a mirror
by 90° nearly without any loss.
Measuring range
