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Microplate Luminometer Structure of the Software
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4.5 Special Functions when Using Injectors
Initialization Prior to the first measurement (after starting the programs), you
must initialize the injector unit by clicking the Initialize button.
Otherwise, no measurement can be started. Then you are
prompted to place a plastic tub into the microplate loading com-
partment (instead of a microplate) to collect any spilled liquid. As
soon as you have placed the plastic tub, click <OK>. The end of
the initialization process is again indicated in a window, while
the pumps perform several lifting motions.
Take the plastic tub out and insert the microplate to be meas-
ured; then click <OK> in the message window. Start the meas-
urement by clicking the green signal light.
Priming Washing and priming of the injector tubings is possible only
when the injector unit has been initialized. Make sure you have
placed an empty plastic tub in the microplate loading compart-
ment to collect spilled liquid!
Click on the Prime button to open the Parameters for Priming
Injectors dialog box.
Select the injector (Inj. 1 or Inj 2).
For each injector, enter the volume/strokes (ml) and the number
of strokes.
The programmed priming process starts as soon as you click
<OK>. When the priming process is finished you can start the
measurement using the selected measurement protocol.
Stopping the Priming Process
You may stop a started priming process any time by clicking the
Stop Priming button.